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Addgene

pLYS1-EMRE/RNAi resistant
(Plasmid #50056)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 50056 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLYS1
  • Backbone size w/o insert (bp) 7330
  • Total vector size (bp) 7651
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    EMRE-RNAi resistant
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    321
  • Mutation
    RNAi resistant, silent mutant
  • GenBank ID
    NM_033318
  • Entrez Gene
    SMDT1 (a.k.a. C22orf32, DDDD, EMRE)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nhe1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer custom
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLYS1-EMRE/RNAi resistant was a gift from Vamsi Mootha (Addgene plasmid # 50056 ; http://n2t.net/addgene:50056 ; RRID:Addgene_50056)
  • For your References section:

    EMRE is an Essential Component of the Mitochondrial Calcium Uniporter Complex. Sancak Y, Markhard AL, Kitami T, Kovacs-Bogdan E, Kamer KJ, Udeshi ND, Carr SA, Chaudhuri D, Clapham DE, Li AA, Calvo SE, Goldberger O, Mootha VK. Science. 2013 Nov 14. 10.1126/science.1242993 PubMed 24231807