pLYS1-EMRE/RNAi resistant
(Plasmid
#50056)
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PurposeExpression of human EMRE in mammalian cells with silent mutation making it resistant to RNAi
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 50056 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLYS1
- Backbone size w/o insert (bp) 7330
- Total vector size (bp) 7651
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL10 Gold
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameEMRE-RNAi resistant
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SpeciesH. sapiens (human)
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Insert Size (bp)321
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MutationRNAi resistant, silent mutant
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GenBank IDNM_033318
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Entrez GeneSMDT1 (a.k.a. C22orf32, DDDD, EMRE)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Nhe1 (not destroyed)
- 3′ cloning site EcoR1 (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer custom (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLYS1-EMRE/RNAi resistant was a gift from Vamsi Mootha (Addgene plasmid # 50056 ; http://n2t.net/addgene:50056 ; RRID:Addgene_50056) -
For your References section:
EMRE is an Essential Component of the Mitochondrial Calcium Uniporter Complex. Sancak Y, Markhard AL, Kitami T, Kovacs-Bogdan E, Kamer KJ, Udeshi ND, Carr SA, Chaudhuri D, Clapham DE, Li AA, Calvo SE, Goldberger O, Mootha VK. Science. 2013 Nov 14. 10.1126/science.1242993 PubMed 24231807