pLYS1-EMRE/RNAi resistant
(Plasmid #50056)

• Purpose: Expression of human EMRE in mammalian cells with silent mutation making it resistant to RNAi
• Depositing Lab: Vamsi Mootha
• Publication: Sancak et al Science. 2013 Nov 14.

Backbone

• Vector backbone: pLYS1
• Backbone size w/o insert (bp): 7330
• Total vector size (bp): 7651
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: EMRE-RNAi resistant
• Species: H. sapiens (human)
• Insert Size (bp): 321
• Mutation: RNAi resistant, silent mutant
• GenBank ID: NM_033318
• Entrez Gene: SMDT1 (a.k.a. C22orf32, DDDD, EMRE)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nhe1 (not destroyed)
• 3′ cloning site: EcoR1 (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: custom

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry