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Addgene

ATSR
(Plasmid #50052)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 50052 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLEXm-LIC
  • Backbone size w/o insert (bp) 4500
  • Total vector size (bp) 5300
  • Modifications to backbone
    The vector is modified for LIC cloning, but the insert can also be extracted using 5' EcoR I/ Nhe I/sal I/BsiW1, and 3' Spe I, Asc I/BssH II/ Not I/Xho I.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MIC2-ATSR
  • Alt name
    MIC2(67-337)
  • Species
    toxoplasma gondii
  • Insert Size (bp)
    810
  • Mutation
    S158A
  • GenBank ID
    AAB63303
  • Promoter Chick beta-actin promoter
  • Tag / Fusion Protein
    • HIS (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer TAGAGCCTCTGCTAACCATGTTCATGCC
  • 3′ sequencing primer CCCCATAATTTTTGGCAGAGGG
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. David Sibley Washington University School of Medicine

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ATSR was a gift from Timothy Springer (Addgene plasmid # 50052 ; http://n2t.net/addgene:50052 ; RRID:Addgene_50052)
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