MIC2
(Plasmid #50050)

• Purpose: expresses toxoplasma gondii MIC2 extracellular domain in mammalian cells
• Depositing Lab: Timothy Springer
• Publication: Springer lab MIC2 plasmid (unpublished)

Backbone

• Vector backbone: pIRES2-EGFP
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 7400
• Modifications to backbone: The vector is modified for LIC cloning, but the insert can also be extracted using 5' Nhe I/sal I/BsiW1, and 3' Spe I, Asc I/BssH II.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: MIC2
• Alt name: TgMIC2
• Alt name: TRAP Homolog
• Alt name: MIC2(30-679)
• Species: toxoplasma gondii
• Insert Size (bp): 2000
• Mutation: S158A, N357S, N463S, N470D
• GenBank ID: AAB63303
• Promoter: CMV
• Tags / Fusion Proteins:
  • TEV (C terminal on insert)
  • SBP (C terminal on insert)
  • HIS (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: IRES-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. David Sibley Washington University School of Medicine

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene has not been unable to verify the N357S, N463S, N470D mutations in the MIC2 sequence.