pfTRAP
(Plasmid #50048)

• Purpose: Expresses plasmodium falciparum TRAP adhesion domains (26-299) in mammalian cells
• Depositing Lab: Timothy Springer
• Publication: Song et al Proc Natl Acad Sci U S A. 2012 Dec 26;109(52):21420-5. doi: 10.1073/pnas.1218581109. Epub 2012 Dec 11.

Backbone

• Vector backbone: pIRES2-EGFP
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 6200
• Modifications to backbone: The vector is modified for LIC cloning, but the insert can also be extracted using 5' Nhe I/sal I/BsiW1, and 3' Spe I, Asc I/BssH II.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: plamodium falciparum TRAP
• Alt name: pfTRAP(26-299)
• Species: Synthetic
• Insert Size (bp): 850
• Mutation: C55G, N132S
• GenBank ID: AAA29775
• Entrez Gene: TRAP (a.k.a. PF13_0201, SSP-2, SSP2)
• Promoter: CMV
• Tag / Fusion Protein:
  • 6Xhis tag (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: IRES-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry