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Addgene

pvTRAP
(Plasmid #50047)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 50047 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pIRES2-EGFP
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 6200
  • Modifications to backbone
    The vector is modified for LIC cloning, but the insert can also be extracted using 5' Nhe I/sal I/BsiW1, and 3' Spe I, Asc I/BssH II.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    plasmodium vivax TRAP
  • Alt name
    PvTRAP(25-283)
  • Species
    Synthetic
  • Insert Size (bp)
    800
  • Mutation
    S42Q, N91S, N128S, S180R
  • GenBank ID
    AAC97484
  • Promoter cmv
  • Tag / Fusion Protein
    • 6Xhis tag (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pvTRAP was a gift from Timothy Springer (Addgene plasmid # 50047 ; http://n2t.net/addgene:50047 ; RRID:Addgene_50047)
  • For your References section:

    Shape change in the receptor for gliding motility in Plasmodium sporozoites. Song G, Koksal AC, Lu C, Springer TA. Proc Natl Acad Sci U S A. 2012 Dec 26;109(52):21420-5. doi: 10.1073/pnas.1218581109. Epub 2012 Dec 11. 10.1073/pnas.1218581109 PubMed 23236185