pvTRAP
(Plasmid
#50047)
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Purposeexpresses plasmodium vivax TRAP adhesion domains (25-283) in mammalian cells
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 50047 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepIRES2-EGFP
- Backbone size w/o insert (bp) 5400
- Total vector size (bp) 6200
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Modifications to backboneThe vector is modified for LIC cloning, but the insert can also be extracted using 5' Nhe I/sal I/BsiW1, and 3' Spe I, Asc I/BssH II.
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameplasmodium vivax TRAP
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Alt namePvTRAP(25-283)
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SpeciesSynthetic
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Insert Size (bp)800
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MutationS42Q, N91S, N128S, S180R
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GenBank IDAAC97484
- Promoter cmv
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Tag
/ Fusion Protein
- 6Xhis tag (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer IRES-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pvTRAP was a gift from Timothy Springer (Addgene plasmid # 50047 ; http://n2t.net/addgene:50047 ; RRID:Addgene_50047) -
For your References section:
Shape change in the receptor for gliding motility in Plasmodium sporozoites. Song G, Koksal AC, Lu C, Springer TA. Proc Natl Acad Sci U S A. 2012 Dec 26;109(52):21420-5. doi: 10.1073/pnas.1218581109. Epub 2012 Dec 11. 10.1073/pnas.1218581109 PubMed 23236185