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pRS303-pGAL-HA-TMSec66-SrtA (pKS105)
(Plasmid #50033)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 50033 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS303
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 5500
  • Modifications to backbone
    Insertion of pGAL promoter (SacI/XbaI)
  • Vector type
    Yeast Expression
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SrtA(strep)
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
    658
  • Promoter pGAL
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CGGTTTGTATTACTTCTTATTCAAATGTAATAAAAGTATC
  • 3′ sequencing primer CCTAGACTTCAGGTTGTCTAACTCCTTC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS303-pGAL-HA-TMSec66-SrtA (pKS105) was a gift from Hidde Ploegh (Addgene plasmid # 50033 ; http://n2t.net/addgene:50033 ; RRID:Addgene_50033)

  • For your References section:

    Protein ligation in living cells using sortase. Strijbis K, Spooner E, Ploegh HL. Traffic. 2012 Jun;13(6):780-9. doi: 10.1111/j.1600-0854.2012.01345.x. Epub 2012 Mar 23. 10.1111/j.1600-0854.2012.01345.x PubMed 22348280
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