pCMV3flag8HOIL-1L
(Plasmid #50016)

• Purpose: Mammalian expression of human HOIL-1L (RBCK1) with a c-terminal 3xFLAG tag, and hygromycin selection.
• Depositing Lab: Martin Dorf
• Publication: Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2013.133.

Backbone

• Vector backbone: pCMV-3Tag-8
• Backbone manufacturer: Agilent (Stratagene)
• Backbone size w/o insert (bp): 5171
• Total vector size (bp): 6710
• Modifications to backbone: Replaced with new MCS: XhoI-Insert[(ccacgccag(5'UT)-Gene CDS(1530bp no Stop)]-NotI-ClaI-3xFLAG
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RanBP-type and C3HC4-type zinc finger containing 1 HOIL-1L
• Alt name: HOIL-1L
• Alt name: RBCK1
• Alt name: HOIL1
• Species: H. sapiens (human)
• Insert Size (bp): 1539
• GenBank ID: NM_031229.2
• Entrez Gene: RBCK1 (a.k.a. C20orf18, HOIL-1, HOIL1, PBMEI, PGBM1, RBCK2, RNF54, UBCE7IP3, XAP3, XAP4, ZRANB4)
• Promoter: CMV
• Tag / Fusion Protein:
  • 3 x FLAG (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Open Biosystems, Thermo Scientific: IHS1380-97433477 Incyte Full Length Human cDNA Clone, Clone Id: LIFESEQ2312159
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry