pCMV3flag8HOIP
(Plasmid #50015)

• Purpose: Mammalian expression of human HOIP (RNF31) with a c-terminal 3xFLAG tag, and hygromycin selection.
• Depositing Lab: Martin Dorf
• Publication: Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2013.133.

Backbone

• Vector backbone: pCMV-3Tag-8
• Backbone manufacturer: Agilent (Stratagene)
• Backbone size w/o insert (bp): 5191
• Total vector size (bp): 8423
• Modifications to backbone: Replaced with new MCS: XhoI-HindIII-BamHI-NotI-Insert[(TGACTCCTGCCTCAGG(5'UT)-Gene CDS(no Stop)]-ClaI -3xFLAG
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ring finger protein 31
• Alt name: HOIP
• Alt name: RNF31
• Alt name: E3 ubiquitin-protein ligase RNF31
• Species: H. sapiens (human)
• Insert Size (bp): 3232
• GenBank ID: BC012077 NM_017999
• Entrez Gene: RNF31 (a.k.a. HOIP, IMD115, Paul, ZIBRA)
• Promoter: CMV
• Tag / Fusion Protein:
  • 3 x FLAG (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: ClaI (not destroyed)
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Open Biosystems / Thermo Scientific MHS1011-76068 Human MGC Verified FL cDNA (IRAU)
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry