pCMV3flag8SHARPIN
(Plasmid #50014)

• Purpose: Mammalian expression of human SHARPIN with a c-terminal 3xFLAG tag, and hygromycin selection.
• Depositing Lab: Martin Dorf
• Publication: Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2013.133.

Backbone

• Vector backbone: pCMV-3Tag-8
• Backbone manufacturer: Agilent (Stratagene)
• Backbone size w/o insert (bp): 5171
• Total vector size (bp): 6339
• Modifications to backbone: Replaced with new MCS: XhoI-Insert[ACCGGAG(5'UT)-Gene CDS(1164bp no Stop)]-NotI-Cla - 3 x FLAG
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SHANK-associated RH domain interactor
• Alt name: Sharpin
• Alt name: SIPL1
• Species: H. sapiens (human)
• Insert Size (bp): 1168
• GenBank ID:
• Entrez Gene: SHARPIN (a.k.a. SIPL1)
• Promoter: CMV
• Tag / Fusion Protein:
  • 3 x FLAG (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (destroyed during cloning)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMVforward
• 3′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: DF/HCC DNA Resource Core, 240 Longwood Avenue Seeley Mudd 228 Boston, MA 02115 (617) 432-7708 Plasmid ID HsCD00332474
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry