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Addgene

pAW8ENdeI-c3HA-4XDSR
(Plasmid #49869)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49869 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC
  • Vector type
    Cre/Lox
  • Selectable markers
    LEU2
  • Tag / Fusion Protein
    • 3XHA (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid contains a wild-type loxP site and a mutant loxP site termed loxM3. Addgene's mapping software can not differentiate between the two sites and identifies the loxM3 site as loxP. The locations of the loxP and loxM3 sites are:

loxP = 3859-3892
loxM3 = 4173-4206

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAW8ENdeI-c3HA-4XDSR was a gift from Tony Carr (Addgene plasmid # 49869 ; http://n2t.net/addgene:49869 ; RRID:Addgene_49869)

  • For your References section:

    Optimisation of the Schizosaccharomyces pombe urg1 Expression System. Watson AT, Daigaku Y, Mohebi S, Etheridge TJ, Chahwan C, Murray JM, Carr AM. PLoS One. 2013 Dec 20;8(12):e83800. doi: 10.1371/journal.pone.0083800. PubMed 24376751
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