pcDNA3.2-GJA1-20K-V5
(Plasmid #49859)

• Purpose: Encodes C-terminal fragment of human connexin 43 initiating at M213, with M281 and M320 mutated to L, and a V5 C-terminal fusion tag
• Depositing Lab: Robin Shaw
• Publication: Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13)00578-0. doi: 10.1016/j.celrep.2013.10.009.

Backbone

• Vector backbone: pcDNA3.2/V5-DEST
• Backbone manufacturer: Life Technologies
• Backbone size w/o insert (bp): 7711
• Total vector size (bp): 8257
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GJA1-20k
• Alt name: GJA1
• Alt name: Connexin 43
• Species: H. sapiens (human)
• Insert Size (bp): 510
• Mutation: GJA1-20k fragment corresponds to residues 213-382 of Connexin 43, no stop codon to allow for c-terminal fusion
• GenBank ID: NM_000165.3
• Entrez Gene: GJA1 (a.k.a. AVSD3, CMDR, CX43, EKVP, EKVP3, GJAL, HLHS1, HSS, ODDD, PPKCA)
• Promoter: CMV
• Tag / Fusion Protein:
  • V5 (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV
• 3′ sequencing primer: V5

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry