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pcDNA3.2-Cx43-M100L-siResist
(Plasmid #49854)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49854 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.2/V5-DEST
  • Backbone manufacturer
    Life Technologies
  • Backbone size w/o insert (bp) 7711
  • Total vector size (bp) 8893
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Connexin 43
  • Alt name
    GJA1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1146
  • Mutation
    Methionine100 mutated to Leucine. Contains several wobble mutations to confer resistance to siRNA
  • GenBank ID
    NM_000165.3
  • Entrez Gene
    GJA1 (a.k.a. AVSD3, CMDR, CX43, EKVP, EKVP3, GJAL, HLHS1, HSS, ODDD, PPKCA)
  • Promoter CMV

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.2-Cx43-M100L-siResist was a gift from Robin Shaw (Addgene plasmid # 49854 ; http://n2t.net/addgene:49854 ; RRID:Addgene_49854)

  • For your References section:

    Autoregulation of Connexin43 Gap Junction Formation by Internally Translated Isoforms. Smyth JW, Shaw RM. Cell Rep. 2013 Nov 5. pii: S2211-1247(13)00578-0. doi: 10.1016/j.celrep.2013.10.009. 10.1016/j.celrep.2013.10.009 PubMed 24210816
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