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hSRAP.TWIN1.a111-215
(Plasmid #49820)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49820 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTWIN1
  • Backbone manufacturer
    New England Biolabs
  • Backbone size w/o insert (bp) 7375
  • Total vector size (bp) 6970
  • Modifications to backbone
    cloning deleted fragment of original vector
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Growth instructions
    BL21(DE3) should be used for protein expression.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    steroid receptor RNA activator protein aa 111-215
  • Alt name
    SRAP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    315
  • Mutation
    wildtype codon-optimized for E. coli expression
  • Entrez Gene
    SRA1 (a.k.a. SRA, SRAP, STRAA1, pp7684)
  • Promoter T7
  • Tag / Fusion Protein
    • intein & chitin binding domain (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer none
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hSRAP.TWIN1.a111-215 was a gift from Thomas Cech (Addgene plasmid # 49820 ; http://n2t.net/addgene:49820 ; RRID:Addgene_49820)

  • For your References section:

    Structure and function of steroid receptor RNA activator protein, the proposed partner of SRA noncoding RNA. McKay DB, Xi L, Barthel KK, Cech TR. J Mol Biol. 2014 Apr 17;426(8):1766-85. doi: 10.1016/j.jmb.2014.01.006. Epub 2014 Jan 30. 10.1016/j.jmb.2014.01.006 PubMed 24486609
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