hSRAP.TWIN1.a13-69
(Plasmid #49815)

• Purpose: expression vector for SRAP(13-69) in pTWIN1 vector
• Depositing Lab: Thomas Cech
• Publication: McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85. doi: 10.1016/j.jmb.2014.01.006. Epub 2014 Jan 30.

Backbone

• Vector backbone: pTWIN1
• Backbone manufacturer: New England Biolabs
• Backbone size w/o insert (bp): 7375
• Total vector size (bp): 6826
• Modifications to backbone: cloning deleted fragment of original vector
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Growth instructions: BL21(DE3) should be used for protein expression.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: steroid receptor RNA activator protein aa 13-69
• Alt name: SRAP
• Species: H. sapiens (human)
• Insert Size (bp): 171
• Mutation: wildtype codon-optimized for E. coli expression
• Entrez Gene: SRA1 (a.k.a. SRA, SRAP, STRAA1, pp7684)
• Promoter: T7
• Tag / Fusion Protein:
  • intein & chitin binding domain (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: SpeI (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: none

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry