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pCDM4-GLY
(Plasmid #49808)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49808 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDM4
  • Backbone size w/o insert (bp) 3810
  • Total vector size (bp) 12630
  • Modifications to backbone
    See paper.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Streptomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    phosphoglycerate kinase
  • Alt name
    pgk
  • Species
    Escherichia coli
  • Insert Size (bp)
    1164
  • GenBank ID
    CDJ73916.1
  • Promoter T7

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site AvrII (destroyed during cloning)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer GTCTGTAATTAAGATGACCGATCTGG
  • 3′ sequencing primer CTTCTTAGCGCGCTCTTCG
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    glyceraldehyde-3-phosphate dehydrogenase
  • Alt name
    gapA
  • Species
    E. coli K-12
  • Insert Size (bp)
    996
  • GenBank ID
    CDJ72173.1
  • Promoter T7

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site AvrII (destroyed during cloning)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer GTAGGTATCAACGGTTTTGGCC
  • 3′ sequencing primer GGAGATGTGAGCGATCAGGTC
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    pyruvate dehydrogenase subunit E1
  • Alt name
    aceE
  • Species
    E. coli K-12
  • Insert Size (bp)
    2664
  • GenBank ID
    CDJ70695.1
  • Promoter T7

Cloning Information for Gene/Insert 3

  • Cloning method Restriction Enzyme
  • 5′ cloning site AvrII (destroyed during cloning)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer GTCAGAACGTTTCCCAAATGACG
  • 3′ sequencing primer CCAGACGCGGGTTAACTTTATC
    • (Common Sequencing Primers)

Gene/Insert 4

  • Gene/Insert name
    dihydrolipoamide acetyltransferase
  • Alt name
    aceF
  • Species
    E. coli
  • Insert Size (bp)
    1893
  • GenBank ID
    CDJ70696.1
  • Promoter T7

Cloning Information for Gene/Insert 4

  • Cloning method Restriction Enzyme
  • 5′ cloning site AvrII (destroyed during cloning)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer GGCTATCGAAATCAAAGTACC
  • 3′ sequencing primer CATCACCAGACGGCGAATG
    • (Common Sequencing Primers)

Gene/Insert 5

  • Gene/Insert name
    dihydrolipoamide dehydrogenase
  • Alt name
    lpdA
  • Species
    E. coli K-12
  • Insert Size (bp)
    1145
  • GenBank ID
    CDJ70697.1
  • Promoter T7

Cloning Information for Gene/Insert 5

  • Cloning method Restriction Enzyme
  • 5′ cloning site AvrII (destroyed during cloning)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer CTCAGGTCGTGGTACTTGG
  • 3′ sequencing primer CTTCTTCTTCGCTTTCGGGTTC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDM4-GLY was a gift from Mattheos Koffas (Addgene plasmid # 49808 ; http://n2t.net/addgene:49808 ; RRID:Addgene_49808)

  • For your References section:

    Modular optimization of multi-gene pathways for fatty acids production in E. coli. Xu P, Gu Q, Wang W, Wong L, Bower AG, Collins CH, Koffas MA. Nat Commun. 2013;4:1409. doi: 10.1038/ncomms2425. 10.1038/ncomms2425 PubMed 23361000
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