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Purposeexpresses UVR8 fused to GFP with a prenylation motif for membrane targeting
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 49805 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP N1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameUVR8-GFPCaax
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SpeciesA. thaliana (mustard weed)
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Insert Size (bp)2142
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Entrez GeneUVR8 (a.k.a. AT5G63860, MGI19.7, MGI19_7, UVB-RESISTANCE 8)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
UVR8-GFPCaaX was a gift from Matthew Kennedy (Addgene plasmid # 49805 ; http://n2t.net/addgene:49805 ; RRID:Addgene_49805) -
For your References section:
A light-triggered protein secretion system. Chen D, Gibson ES, Kennedy MJ. J Cell Biol. 2013 May 13;201(4):631-40. doi: 10.1083/jcb.201210119. 10.1083/jcb.201210119 PubMed 23671313