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Purposeexpresses VSVG fused to UVR8 for light-triggered trafficking
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 49800 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepC4-Fm2E
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Backbone manufacturerAriad
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVSVG-YFP-2xUVR8
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Insert Size (bp)5064
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MutationVSVG contains ts045 mutations
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Tags
/ Fusion Proteins
- YFP (C terminal on insert)
- HA (C terminal on insert)
- MYC (C terminal on insert)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
VSVG-YFP-2xUVR8 was a gift from Matthew Kennedy (Addgene plasmid # 49800 ; http://n2t.net/addgene:49800 ; RRID:Addgene_49800) -
For your References section:
A light-triggered protein secretion system. Chen D, Gibson ES, Kennedy MJ. J Cell Biol. 2013 May 13;201(4):631-40. doi: 10.1083/jcb.201210119. 10.1083/jcb.201210119 PubMed 23671313