pCOM4
(Plasmid
#49798)
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Purpose(Empty Backbone) ePathBrick vector for pathway engineering in E. coli.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 49798 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCOLA-Duet
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Backbone manufacturerNovagen
- Backbone size (bp) 5000
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Modifications to backboneAvrII was inserted upstream of the T7 promoter, XbaI was inserted between the operator (lacO) and ribosome binding site (RBS), SpeI was between the multiple-cloning site (MCS) and T7 terminator, and NheI was placed downstream of the T7 terminator
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Vector typeBacterial Expression
- Promoter T7
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Tag
/ Fusion Protein
- S-tag (C terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Cloning Information
- Cloning method Restriction Enzyme
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The discrepancies between the Addgene QC sequence and full sequence do not affect plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCOM4 was a gift from Mattheos Koffas (Addgene plasmid # 49798 ; http://n2t.net/addgene:49798 ; RRID:Addgene_49798) -
For your References section:
ePathBrick: a synthetic biology platform for engineering metabolic pathways in E. coli. Xu P, Vansiri A, Bhan N, Koffas MA. ACS Synth Biol. 2012 Jul 20;1(7):256-66. doi: 10.1021/sb300016b. Epub 2012 May 4. 10.1021/sb300016b PubMed 23651248
