pCDM4
(Plasmid #49796)

• Purpose: (Empty Backbone) ePathBrick vector for pathway engineering in E. coli.
• Depositing Lab: Mattheos Koffas
• Publication: Xu et al ACS Synth Biol. 2012 Jul 20;1(7):256-66. doi: 10.1021/sb300016b. Epub 2012 May 4.

Backbone

• Vector backbone: pCDFDuet
• Backbone manufacturer: Novagen
• Backbone size (bp): 5000
• Modifications to backbone: AvrII was inserted upstream of the T7 promoter, XbaI was inserted between the operator (lacO) and ribosome binding site (RBS), SpeI was between the multiple-cloning site (MCS) and T7 terminator, and NheI was placed downstream of the T7 terminator
• Vector type: Bacterial Expression
• Promoter: T7
• Tag / Fusion Protein:
  • S-tag (C terminal on insert)

Growth in Bacteria

• Bacterial Resistance(s): Streptomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Cloning Information

• Cloning method: Restriction Enzyme

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry