pUC6
(Plasmid
#49793)
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Purpose(Empty Backbone) E. coli cloning vector
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 49793 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBR322
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Backbone manufacturerATCC
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Modifications to backboneThe plasmid constructions are derived from pBR322 (Bolivar et al., 1977). The first derivative is pURl (Ruther, 1980), a mini pBR322 containing nucleotides 2067 to 4362 map position and the ampicillin-resistance gene. The PstI site CTGCAG (3612) was changed to CTACAG to yield pUC3. The HincII site GTCAAC (3908) was changed to GTCAAT to yield pUC5. The AccI site (2246) was deleted by BAL31 treatment of yield pUC6.
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Vector typecloning vector
- Promoter none
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC6 was a gift from Joachim Messing (Addgene plasmid # 49793 ; http://n2t.net/addgene:49793 ; RRID:Addgene_49793) -
For your References section:
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Vieira J, Messing J. Gene. 1982 Oct;19(3):259-68. 10.1016/0378-1119(82)90015-4 PubMed 6295879