FH-TET1-pEF
(Plasmid #49792)

• Purpose: This plasmid expresses the full-length human TET1 gene with a Flag and HA tag.
• Depositing Lab: Anjana Rao
• Publication: Tahiliani et al Science. 2009 May 15;324(5929):930-5. Epub 2009 Apr 16.

Backbone

• Vector backbone: pEF1a
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 6200
• Total vector size (bp): 12692
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Tet methylcytosine dioxygenase 1
• Alt name: TET1
• Species: H. sapiens (human)
• Insert Size (bp): 6492
• Entrez Gene: TET1 (a.k.a. CXXC6, LCX, bA119F7.1)
• Promoter: EF-1alpha
• Tags / Fusion Proteins:
  • Flag (N terminal on insert)
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: BGH Reverse

Resource Information

• Articles Citing this Plasmid:
  • 21 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that compared to GenBank reference sequence NM_030625.2, the TET1 insert in this plasmid contains a SNP at amino acid 1123. The resulting I1223M amino acid change does not affect TET1 expression or catalytic activity.