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Addgene

Golgi-DKAR
(Plasmid #49790)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49790 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    SURE
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    D kinase activity reporter
  • Alt name
    eNOS-CFP-FHA2-peptide-YFP
  • Species
    Synthetic
  • Promoter CMV
  • Tags / Fusion Proteins
    • eNOS(1-33) (N terminal on insert)
    • CFP (N terminal on insert)
    • YFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (not destroyed)
  • 3′ cloning site Xba I (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Golgi-targeted, FRET-based PKD activation reporter (DKAR). Contains a PKD-specific substrate and phosphoamino acid-binding domain linking CFP and YFP. Upon phosphorylation by PKD, FRET is reduced.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Golgi-DKAR was a gift from Alexandra Newton (Addgene plasmid # 49790 ; http://n2t.net/addgene:49790 ; RRID:Addgene_49790)

  • For your References section:

    Calcium transduces plasma membrane receptor signals to produce diacylglycerol at Golgi membranes. Kunkel MT, Newton AC. J Biol Chem. 2010 Jul 23;285(30):22748-52. doi: 10.1074/jbc.C110.123133. Epub 2010 Jun 2. 10.1074/jbc.C110.123133 PubMed 20519514
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