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Purpose(Empty Backbone) E. coli cloning vector (KanR, low copy, blue/white selection)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 49789 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC21 (Addgene plasmid #49787)
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Backbone manufacturerMessing Lab
- Backbone size (bp) 2135
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Modifications to backboneThe lac region and MCS are identical to pUC21, The KmR gene is from Tn905 and the ori is P15A from pACYC 177.
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Vector typecloning vector
- Promoter lacZ
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOK12 was a gift from Joachim Messing (Addgene plasmid # 49789 ; http://n2t.net/addgene:49789 ; RRID:Addgene_49789) -
For your References section:
New pUC-derived cloning vectors with different selectable markers and DNA replication origins. Vieira J, Messing J. Gene. 1991 Apr;100:189-94. 10.1016/0378-1119(91)90365-I PubMed 1905257
Map uploaded by the depositor.
