pcDNA3-Flag-YOD1
(Plasmid #49784)

• Purpose: Express YOD1 with a N-terminal Flag-tag in mammalian cells
• Depositing Lab: Christian Schlieker
• Publication: Ernst et al Mol Cell. 2009 Oct 9;36(1):28-38. doi: 10.1016/j.molcel.2009.09.016.

Backbone

• Vector backbone: pcDNA3.1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: YOD1
• Species: H. sapiens (human)
• Mutation: WT
• Entrez Gene: YOD1 (a.k.a. DUBA8, OTUD2, PRO0907)
• Tag / Fusion Protein:
  • Flag-tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Hind III (not destroyed)
• 3′ cloning site: Xba I (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: pcDNA3 rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry