pYes2-TaPCS1
(Plasmid
#49767)
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PurposeExpresses wheat phytochelatin synthase (TaPCS1) in Saccharomyces Cerevisiae
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 49767 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepYes2
- Backbone size w/o insert (bp) 6000
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTaPCS1
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Alt namewheat phytochelatin synthase
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SpeciesTriticum aestivum
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Entrez GenePCS1
- Promoter GAL1
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer GAL1
- 3′ sequencing primer CYC1 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDr. Stephan Clemens
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYes2-TaPCS1 was a gift from Julian Schroeder (Addgene plasmid # 49767 ; http://n2t.net/addgene:49767 ; RRID:Addgene_49767) -
For your References section:
Tolerance to toxic metals by a gene family of phytochelatin synthases from plants and yeast. Clemens S, Kim EJ, Neumann D, Schroeder JI. EMBO J. 1999 Jun 15;18(12):3325-33. 10.1093/emboj/18.12.3325 PubMed 10369673