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Addgene

pYes2-AtPCS1
(Plasmid #49766)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49766 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYes2
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    AtPCS1
  • Alt name
    Arabidopsis Phytochelatin synthase
  • Species
    A. thaliana (mustard weed)
  • Entrez Gene
    PCS1 (a.k.a. AT5G02190, ARABIDOPSIS THALIANA ASPARTIC PROTEASE 38, ATASP38, EMB24, EMBRYO DEFECTIVE 24, PHYTOCHELATIN SYNTHASE 1, PROMOTION OF CELL SURVIVAL 1, T7H20.240, T7H20_240)
  • Promoter GAL1

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Stephan Clemens

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYes2-AtPCS1 was a gift from Julian Schroeder (Addgene plasmid # 49766 ; http://n2t.net/addgene:49766 ; RRID:Addgene_49766)

  • For your References section:

    Tolerance to toxic metals by a gene family of phytochelatin synthases from plants and yeast. Clemens S, Kim EJ, Neumann D, Schroeder JI. EMBO J. 1999 Jun 15;18(12):3325-33. 10.1093/emboj/18.12.3325 PubMed 10369673
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