pBI121-Pro-Adh::TaPCS1:cmyc
(Plasmid #49765)

• Purpose: Expresses wheat phytochelatin synthase (TaPCS1) in plant expression vector
• Depositing Lab: Julian Schroeder
• Publication: Gong et al Proc Natl Acad Sci U S A. 2003 Aug 19;100(17):10118-23. Epub 2003 Aug 8.

Backbone

• Vector backbone: pBI121
• Backbone size w/o insert (bp): 13000
• Vector type: Plant expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: TaPCS1
• Alt name: Phytochelatin synthase
• Species: Triticum aestivum
• GenBank ID: AF093752.1
• Entrez Gene: PCS1
• Promoter: Adh1
• Tag / Fusion Protein:
  • c-myc (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: SacI (unknown if destroyed)
• 5′ sequencing primer: lac-promoter (5'-CACTTTATGCTTCCGGCTCG-3')
• 3′ sequencing primer: M13-F20 (5'-TGTAAAACGACGGCCAGT-3')

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry