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Addgene

pTH-NI(A)-mCherry
(Plasmid #49626)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49626 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA4/TO
  • Backbone manufacturer
    Invitrogen
  • Backbone size (bp) 5500
  • Vector type
    Mammalian Expression, TALEN
  • Promoter CMV
  • Selectable markers
    Hygromycin
  • Tag / Fusion Protein
    • mCherry (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This destination vector for TALEs is compatible with the Zhang Lab TALE Toolbox.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTH-NI(A)-mCherry was a gift from Thoru Pederson (Addgene plasmid # 49626 ; http://n2t.net/addgene:49626 ; RRID:Addgene_49626)
  • For your References section:

    Visualization of repetitive DNA sequences in human chromosomes with transcription activator-like effectors. Ma H, Reyes-Gutierrez P, Pederson T. Proc Natl Acad Sci U S A. 2013 Dec 9. 10.1073/pnas.1319097110 PubMed 24324157