pBMN HAYFP-LOV24
(Plasmid #49570)

• Purpose: regulate protein stability with light
• Depositing Lab: Thomas Wandless
• Publication: Bonger et al ACS Chem Biol. 2014 Jan 17;9(1):111-5. doi: 10.1021/cb400755b

Backbone

• Vector backbone: pBMN
• Backbone manufacturer: Garry Nolan
• Backbone size w/o insert (bp): 6943
• Total vector size (bp): 8150
• Vector type: Retroviral
• Selectable markers: HcRed fluorescence behind IRES

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Top10
• Growth instructions: TOP10 cells. Culture at 30C to prevent recombination of LTR and lost insert. Takes >20 hours to get good culture at 30C
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: YFP-LOV24
• Alt name: YFP fused to B-LID
• Species: Avena sativaied from LOV2 domain
• Insert Size (bp): 1203
• Mutation: delete the last 3 aa from AsLOV2, with I532A mutation and RRRG degron
• GenBank ID: AF033096.1
• Promoter: LTR
• Tags / Fusion Proteins:
  • YFP (N terminal on insert)
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NsiI (not destroyed)
• 5′ sequencing primer: Lab 32
• 3′ sequencing primer: LC47

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry