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psicheck-2-Mrpl11-mod
(Plasmid #49569)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49569 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    psicheck-2
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 6273
  • Total vector size (bp) 6368
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mrpl11-mod
  • Species
    M. musculus (mouse)
  • Mutation
    T/A mutation in ATG start site
  • GenBank ID
    NM_025553

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nhe1 (not destroyed)
  • 3′ cloning site Nhe1 (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer psiCHECK2-R (5'-CGAGGTCCGAAGACTCATTT-3')
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    psicheck-2-Mrpl11-mod was a gift from Vamsi Mootha (Addgene plasmid # 49569 ; http://n2t.net/addgene:49569 ; RRID:Addgene_49569)

  • For your References section:

    Upstream open reading frames cause widespread reduction of protein expression and are polymorphic among humans. Calvo SE, Pagliarini DJ, Mootha VK. Proc Natl Acad Sci U S A. 2009 May 5;106(18):7507-12. doi: 10.1073/pnas.0810916106. Epub 2009 Apr 16. 10.1073/pnas.0810916106 PubMed 19372376
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