pSK+ mBMPR-1B DN
(Plasmid #49530)

• Purpose: cloning vector for dominant negative mouse BMP receptor 1B
• Depositing Labs: Lee Niswander, Peter ten Dijke
• Publication: Zou et al Genes Dev. 1997 Sep 1;11(17):2191-203.

Backbone

• Vector backbone: pBluescript SK+
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 2959
• Total vector size (bp): 4500
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BMPR-1B DN
• Alt name: alk-6
• Alt name: BMPR-1B
• Species: M. musculus (mouse)
• Mutation: dominant negative mutation (K231R); also L2V
• Entrez Gene: Bmpr1b (a.k.a. AI385617, ALK-6, AV355320, Acv, Acvrlk6, Al, Alk6, BMPR, BMPR-1B, BMPR-IB, CFK-43, CFK-43a, SKR6)
• Promoter: T3

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The BMPR-1B insert in this plasmid contains a L2V mutation when compared to GenBank reference sequence NP_031586.1. This mutation is not a concern for the function of the plasmid, as described in the associated publication.