pSK+ chickBMPR-1B CA
(Plasmid #49528)

• Purpose: cloning vector for constitutively active chick BMP receptor 1B
• Depositing Labs: Lee Niswander, Peter ten Dijke
• Publication: Varley et al Dev Biol. 1998 Apr 1;196(1):107-18.

Backbone

• Vector backbone: pBluescript SK+
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 2959
• Total vector size (bp): 4500
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BMPR-1B CA
• Alt name: rpk-1
• Alt name: BMPR-1B
• Alt name: alk-6
• Species: G. gallus (chicken)
• Mutation: constitutively active mutation (Q203D); also P2A, M11V and S18G
• Entrez Gene: BMPR1B (a.k.a. RPK-1)
• Promoter: T3

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The BMPR-1B insert in this plasmid contains P2A, M11V and S18G mutations when compared to GenBank reference sequence NP_990463.1. These mutations are not a concern for the function of the plasmid, as described in the associated publication.