pCMV5-hBMPR-1A CA
(Plasmid #49527)

• Purpose: expresses constitutively active human BMP receptor 1A
• Depositing Labs: Lee Niswander, Peter ten Dijke
• Publication: Varley et al Dev Biol. 1998 Apr 1;196(1):107-18.

Backbone

• Vector backbone: pCMV5
• Backbone manufacturer: Dr. David Russell, UTSW
• Backbone size w/o insert (bp): 4657
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: BMPR-1A CA
• Alt name: BMPR-1A
• Alt name: Alk3
• Species: H. sapiens (human)
• Mutation: constitutively active mutation (Q233D); also P2A and K208R
• Entrez Gene: BMPR1A (a.k.a. 10q23del, ACVRLK3, ALK-3, ALK3, BMPR-1A, CD292, SKR5)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: hGH-PA-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The BMPR-1A insert in this plasmid contains P2A and K208R mutations when compared to GenBank reference sequence NP_004320.2. These mutations are not a concern for the function of the plasmid, as described in the associated publication.