pEN41A-T38S38
(Plasmid
#49524)
-
PurposeAmpicillin resistant; entry clone encoding reverse TetR (tetR38) and contains attL4 and attL1 sites for recombination into a Gateway destination vector at the attR4 and attR1 sites
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 49524 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepDO41A
- Backbone size w/o insert (bp) 2728
- Total vector size (bp) 3880
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)Mach1
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameconstitutive TetR38
-
Insert Size (bp)1152
- Promoter Ptb38
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pEN41A-T38S38 was a gift from Dirk Schnappinger (Addgene plasmid # 49524 ; http://n2t.net/addgene:49524 ; RRID:Addgene_49524) -
For your References section:
A genetic strategy to identify targets for the development of drugs that prevent bacterial persistence. Kim JH, O'Brien KM, Sharma R, Boshoff HI, Rehren G, Chakraborty S, Wallach JB, Monteleone M, Wilson DJ, Aldrich CC, Barry CE 3rd, Rhee KY, Ehrt S, Schnappinger D. Proc Natl Acad Sci U S A. 2013 Nov 4. 10.1073/pnas.1315860110 PubMed 24191058