pDE43-MCK
(Plasmid
#49523)
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Purpose(Empty Backbone) Kanamycin and Chloramphenicol resistant; destination vector engineered to integrate into the mycobacteriophage att-L5 site and designed to be used with the multisite Gateway cloning system
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 49523 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDE43-MCK
- Backbone size (bp) 5993
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Vector typeBacterial Expression
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Selectable markersChloramphenicol
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Kanamycin, 25 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DB3.1
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Copy numberLow Copy
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDE43-MCK was a gift from Dirk Schnappinger (Addgene plasmid # 49523 ; http://n2t.net/addgene:49523 ; RRID:Addgene_49523) -
For your References section:
A genetic strategy to identify targets for the development of drugs that prevent bacterial persistence. Kim JH, O'Brien KM, Sharma R, Boshoff HI, Rehren G, Chakraborty S, Wallach JB, Monteleone M, Wilson DJ, Aldrich CC, Barry CE 3rd, Rhee KY, Ehrt S, Schnappinger D. Proc Natl Acad Sci U S A. 2013 Nov 4. 10.1073/pnas.1315860110 PubMed 24191058
Map uploaded by the depositor.
