pGMCKq20-T38S38-0X
(Plasmid
#49522)
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PurposeKanamycin resistant; contains qtag20 and constitutively expresses reverse TetR (tetR38); integrates into the mycobacteriophage att-L5 site
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 49522 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDE43-MCKq20
- Backbone size w/o insert (bp) 4653
- Total vector size (bp) 5805
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Mach1
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameconstitutive TetR38
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Insert Size (bp)1152
- Promoter Ptb38
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGMCKq20-T38S38-0X was a gift from Dirk Schnappinger (Addgene plasmid # 49522 ; http://n2t.net/addgene:49522 ; RRID:Addgene_49522) -
For your References section:
A genetic strategy to identify targets for the development of drugs that prevent bacterial persistence. Kim JH, O'Brien KM, Sharma R, Boshoff HI, Rehren G, Chakraborty S, Wallach JB, Monteleone M, Wilson DJ, Aldrich CC, Barry CE 3rd, Rhee KY, Ehrt S, Schnappinger D. Proc Natl Acad Sci U S A. 2013 Nov 4. 10.1073/pnas.1315860110 PubMed 24191058
Map uploaded by the depositor.
