pGMCtN-TSC10M1-sspB
(Plasmid #49521)

• Purpose: Nourseothricin resistant; expresses sspB under the control of a promoter containing PtetO and constitutively expressses single-chain TetR10 (tsc10); integrates into mycobacteriophage att-Tweety site
• Depositing Lab: Dirk Schnappinger
• Publication: Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

Backbone

• Vector backbone: pDE43-MCtN
• Backbone size w/o insert (bp): 3761
• Total vector size (bp): 6215

Growth in Bacteria

• Bacterial Resistance(s): Nourseothricin (clonNat), 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Mach1
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: TSC10M1-sspB
• Insert Size (bp): 2454

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Contains a fragment amplified by PCR from pTTP1A, a plasmid Dr. Dirk Schnappinger obtained from Dr. Graham Hatfull at University of Pittsburgh under a material transfer agreement. University of Pittsburgh has granted permission to Cornell University for depositing pGMCtN-TSC10M1-sspB and pGMCtK-TSC10M1-sspB into Addgene.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry