pGMEH-T38M-750-luxAB-DAS+4
(Plasmid #49520)

• Purpose: Hygromycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; constitutively expresses reverse TetR (tetR38); replicates episomally
• Depositing Lab: Dirk Schnappinger
• Publication: Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

Backbone

• Vector backbone: pDE43-MEH
• Backbone size w/o insert (bp): 6465
• Total vector size (bp): 9717
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Hygromycin, 200 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Mach1
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: T38M-P750-luxAB-DAS+4
• Insert Size (bp): 3511
• Tag / Fusion Protein:
  • DAS+4 (AANDENYSENYADAS) (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry