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pGMEH-0X750-luxAB-DAS+4
(Plasmid #49519)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49519 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDE43-MEH
  • Backbone size w/o insert (bp) 6465
  • Total vector size (bp) 8802
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Hygromycin, 200 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Mach1
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    P750-luxAB-DAS+4
  • Insert Size (bp)
    2337
  • Promoter P750
  • Tag / Fusion Protein
    • DAS+4 (AANDENYSENYADAS) (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGMEH-0X750-luxAB-DAS+4 was a gift from Dirk Schnappinger (Addgene plasmid # 49519 ; http://n2t.net/addgene:49519 ; RRID:Addgene_49519)

  • For your References section:

    A genetic strategy to identify targets for the development of drugs that prevent bacterial persistence. Kim JH, O'Brien KM, Sharma R, Boshoff HI, Rehren G, Chakraborty S, Wallach JB, Monteleone M, Wilson DJ, Aldrich CC, Barry CE 3rd, Rhee KY, Ehrt S, Schnappinger D. Proc Natl Acad Sci U S A. 2013 Nov 4. 10.1073/pnas.1315860110 PubMed 24191058
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