pGMCK-0X750-luxAB-DAS+4
(Plasmid #49517)

• Purpose: Kanamycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; integrates into the mycobacteriophage att-L5 site
• Depositing Lab: Dirk Schnappinger
• Publication: Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

Backbone

• Vector backbone: pDE43-MCK
• Backbone size w/o insert (bp): 5274
• Total vector size (bp): 7611
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Mach1
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: P750-luxAB-DAS+4
• Insert Size (bp): 2337
• Promoter: P750
• Tag / Fusion Protein:
  • DAS+4 (AANDENYSENYADAS) (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry