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flag-hKLF6-4A (1010)
(Plasmid #49489)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49489 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCI-neo
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5472
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    KFL6-4A
  • Alt name
    Kruppel-like factor 6
  • Alt name
    KFL6
  • Species
    H. sapiens (human)
  • Mutation
    three serines and one tyrosine mutated to alanine at residues 143, 147, 151 and 155 (Alanine amino acids are unable to become phosphorylated)
  • Entrez Gene
    KLF6 (a.k.a. BCD1, CBA1, COPEB, CPBP, GBF, PAC1, ST12, ZF9)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer EBV-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    flag-hKLF6-4A (1010) was a gift from Scott Friedman (Addgene plasmid # 49489 ; http://n2t.net/addgene:49489 ; RRID:Addgene_49489)

  • For your References section:

    GSK3beta phosphorylation of the KLF6 tumor suppressor promotes its transactivation of p21. Lang UE, Kocabayoglu P, Cheng GZ, Ghiassi-Nejad Z, Munoz U, Vetter D, Eckstein DA, Hannivoort RA, Walsh MJ, Friedman SL. Oncogene. 2012 Oct 22. doi: 10.1038/onc.2012.457. 10.1038/onc.2012.457 PubMed 23085750
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