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HA-HIF1a (S668A)
(Plasmid #49463)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49463 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 5433
  • Total vector size (bp) 7938
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Hypoxia inducible factor 1 alpha
  • Alt name
    HIF-1a
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2505
  • Mutation
    Serine 668 to Alanine
  • GenBank ID
    NM_001530
  • Entrez Gene
    HIF1A (a.k.a. HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78)
  • Promoter T7
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (unknown if destroyed)
  • 3′ cloning site ECOR1 (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Addgene Plasmid 18949: HA-HIF1alpha-pcDNA3

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-HIF1a (S668A) was a gift from Wafik El-Deiry (Addgene plasmid # 49463 ; http://n2t.net/addgene:49463 ; RRID:Addgene_49463)

  • For your References section:

    CDK1 stabilizes HIF-1alpha via direct phosphorylation of Ser668 to promote tumor growth. Warfel NA, Dolloff NG, Dicker DT, Malysz J, El-Deiry WS. Cell Cycle. 2013 Oct 25;12(23). 10.4161/cc.26930 PubMed 24189531
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