pBIS-GALkFLP(Trp1)
(Plasmid #49459)

• Purpose: overespression of Flp step-arrest mutant in yeast, TRP1 selection
• Depositing Lab: Marc Gartenberg
• Publication: Tsalik et al Yeast. 1998 Jun 30;14(9):847-52.

Backbone

• Vector backbone: pRS414
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 4788
• Total vector size (bp): 6751
• Vector type: Yeast Expression
• Selectable markers: TRP1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Flp(H305L)
• Alt name: Flp recombinase
• Species: S. cerevisiae (budding yeast)
• Mutation: H305L, ‘step-arrest’ mutant,
• Entrez Gene: flp
• Promoter: GAL10

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: M13-F20
• 3′ sequencing primer: M13R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid expresses a Flp ‘step-arrest’ mutant, Flp H305L, which carries out the first step of recombination, DNA cleavage, but fails to accomplish subsequent strand exchange and religation (Parsons et al.,1988).