pMC6-TEFkFLP(LYS2)
(Plasmid
#49458)
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Purposeoverespression of Flp step-arrest mutant in yeast, LYS2 selection
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 49458 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRS317
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Backbone manufacturerATCC
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Vector typeYeast Expression
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Selectable markersLYS2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFlp(H305L)
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Alt nameFlp recombinase
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SpeciesS. cerevisiae (budding yeast)
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MutationH305L, ‘step-arrest’ mutant,
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Entrez Geneflp
- Promoter TEF1
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMC6-TEFkFLP(LYS2) was a gift from Marc Gartenberg (Addgene plasmid # 49458 ; http://n2t.net/addgene:49458 ; RRID:Addgene_49458)