pRS415-recR
(Plasmid
#49457)
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Purposesingle copy recombinase yeast expression plasmid
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 49457 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRS314
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 6000
- Total vector size (bp) 8653
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namerecR
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Alt nameR-site specific recombinase
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Specieszygosaccharomyces rouxii
- Promoter Gal1
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRS415-recR was a gift from Marc Gartenberg (Addgene plasmid # 49457 ; http://n2t.net/addgene:49457 ; RRID:Addgene_49457) -
For your References section:
Isolation of selected chromatin fragments from yeast by site-specific recombination in vivo. Ansari A, Cheng TH, Gartenberg MR. Methods. 1999 Feb;17(2):104-11. 10.1006/meth.1998.0722 PubMed 10075889
