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Addgene

pRKO
(Plasmid #49456)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49456 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRX1
  • Backbone manufacturer
    Yu X. and Gabriel,A. (1999)
  • Total vector size (bp) 8000
  • Vector type
    Bacterial Expression, Cre/Lox
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    loxP-kanMX-loxP cassette
  • Species
    Synthetic
  • Promoter none

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (destroyed during cloning)
  • 3′ cloning site XhoI (destroyed during cloning)
  • 5′ sequencing primer M13_forward20_primer
  • 3′ sequencing primer M13_Reverse_primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRKO was a gift from Marc Gartenberg (Addgene plasmid # 49456 ; http://n2t.net/addgene:49456 ; RRID:Addgene_49456)

  • For your References section:

    Controlling gene expression in yeast by inducible site-specific recombination. Cheng TH, Chang CR, Joy P, Yablok S, Gartenberg MR. Nucleic Acids Res. 2000 Dec 15;28(24):E108. 10.1093/nar/28.24.e108 PubMed 11121495
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