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Purposeyeast expression of Cre recombinase fused to the estrogen binding domain (EBD), which confers estradiol-dependent control to Cre recombinase
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 49455 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSH62
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Backbone manufacturerGüldener U., et al. (1996)
- Backbone size w/o insert (bp) 7854
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Vector typeYeast Expression, Cre/Lox
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCre-EBD fusion
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SpeciesSynthetic
- Promoter Gal1 promoter
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer Gal1 primer
- 3′ sequencing primer Cyc1 primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid pSH62-EBD was generated by an in vivo recombination approach.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSH62-EBD was a gift from Marc Gartenberg (Addgene plasmid # 49455 ; http://n2t.net/addgene:49455 ; RRID:Addgene_49455) -
For your References section:
Controlling gene expression in yeast by inducible site-specific recombination. Cheng TH, Chang CR, Joy P, Yablok S, Gartenberg MR. Nucleic Acids Res. 2000 Dec 15;28(24):E108. 10.1093/nar/28.24.e108 PubMed 11121495
