Cyto-roGFP
(Plasmid #49435)

• Purpose: Expresses thiol redox-sensitive ratiometric sensor roGFP in cytosol of mammalian cells
• Depositing Lab: Paul Schumacker
• Publication: Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi: 10.1161/CIRCRESAHA.109.206334. Epub 2009 Dec 17.

Backbone

• Vector backbone: VQ Ad5CMV K-NpA
• Backbone manufacturer: ViraQuest Inc.
• Backbone size w/o insert (bp): 5217
• Total vector size (bp): 5970
• Modifications to backbone: None
• Vector type: Mammalian Expression, Adenoviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: roGFP2
• Alt name: Cyto-roGFP2
• Species: Synthetic
• Insert Size (bp): 750
• Mutation: C48S, Q80R, S147C, and Q204C in Clontech's GFP
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: 5'GGCGTGTACGGTGGGAGGTCTATA-3'
• 3′ sequencing primer: 5'-TTAAAAAACCTCCCCACCTCCCCC-3'

Resource Information

• Supplemental Documents:
  • Description of Cyto-roGFP
• A portion of this plasmid was derived from a plasmid made by: We inserted mutations into Clontech's eGFP gene to convert it into roGFP, and then ligated it into a ViraQuest plasmid to permit expression in mammalian cells under the CMV promoter, and also to permit generation of adenovirus by ViraQuest in Iowa.
• Articles Citing this Plasmid:
  • 16 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry