CuGFP-ATG8(416)
(Plasmid #49423)

• Purpose: Expresses ATG8 fused at the N terminus to GFP under the control of the CUP1 promoter.
• Depositing Lab: Daniel Klionsky
• Publication: Wang et al J Biol Chem. 2012 Nov 2;287(45):37964-72. doi: 10.1074/jbc.M112.371591. Epub 2012 Sep 13.

Backbone

• Vector backbone: pRS416
• Backbone size w/o insert (bp): 4898
• Vector type: Bacterial Expression, Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: autophagy-related 8
• Alt name: ATG8
• Alt name: AUT7
• Alt name: APG8
• Species: S. cerevisiae (budding yeast)
• Entrez Gene: ATG8 (a.k.a. YBL078C, APG8, AUT7, CVT5)
• Promoter: CUP1
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XmaI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: NA
• 3′ sequencing primer: NA

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry