pCFD3-dU6:3gRNA Citations (87)

Originally described in: Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila.
Port F, Chen HM, Lee T, Bullock SL Proc Natl Acad Sci U S A. 2014 Jul 7. pii: 201405500. PubMed Journal

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Articles Citing pCFD3-dU6:3gRNA

Articles
Genome editing. The mutagenic chain reaction: a method for converting heterozygous to homozygous mutations. Gantz VM, Bier E. Science. 2015 Apr 24;348(6233):442-4. doi: 10.1126/science.aaa5945. Epub 2015 Mar 19. PubMed
Systematic Evaluation of Drosophila CRISPR Tools Reveals Safe and Robust Alternatives to Autonomous Gene Drives in Basic Research. Port F, Muschalik N, Bullock SL. G3 (Bethesda). 2015 May 20;5(7):1493-502. doi: 10.1534/g3.115.019083. PubMed
A CRISPR/Cas9 mediated point mutation in the alpha 6 subunit of the nicotinic acetylcholine receptor confers resistance to spinosad in Drosophila melanogaster. Zimmer CT, Garrood WT, Puinean AM, Eckel-Zimmer M, Williamson MS, Davies TG, Bass C. Insect Biochem Mol Biol. 2016 Jun;73:62-9. doi: 10.1016/j.ibmb.2016.04.007. Epub 2016 Apr 24. PubMed
Tools and strategies for scarless allele replacement in Drosophila using CRISPR/Cas9. Lamb AM, Walker EA, Wittkopp PJ. Fly (Austin). 2016 Aug 5:1-12. PubMed Associated Plasmids pGEM-WingGFP-tan pGEM-3XP3.RFP-tan
Creating Heritable Mutations in Drosophila with CRISPR-Cas9. Port F, Bullock SL. Methods Mol Biol. 2016;1478:145-160. PubMed
Efficient Screening of CRISPR/Cas9-Induced Events in Drosophila Using a Co-CRISPR Strategy. Kane NS, Vora M, Varre KJ, Padgett RW. G3 (Bethesda). 2017 Jan 5;7(1):87-93. doi: 10.1534/g3.116.036723. PubMed Associated Plasmids pCFD3-ebony
Using CRISPR-Cas9 to Study ERK Signaling in Drosophila. Fores M, Papagianni A, Rodriguez-Munoz L, Jimenez G. Methods Mol Biol. 2017;1487:353-365. PubMed
Probing the canonicity of the Wnt/Wingless signaling pathway. Franz A, Shlyueva D, Brunner E, Stark A, Basler K. PLoS Genet. 2017 Apr 3;13(4):e1006700. doi: 10.1371/journal.pgen.1006700. eCollection 2017 Apr. PubMed
MCTP is an ER-resident calcium sensor that stabilizes synaptic transmission and homeostatic plasticity. Genc O, Dickman DK, Ma W, Tong A, Fetter RD, Davis GW. Elife. 2017 May 9;6. pii: e22904. doi: 10.7554/eLife.22904. PubMed
Patterned cortical tension mediated by N-cadherin controls cell geometric order in the Drosophila eye. Chan EH, Chavadimane Shivakumar P, Clement R, Laugier E, Lenne PF. Elife. 2017 May 24;6. pii: e22796. doi: 10.7554/eLife.22796. PubMed
Novel CRISPR/Cas9 gene drive constructs reveal insights into mechanisms of resistance allele formation and drive efficiency in genetically diverse populations. Champer J, Reeves R, Oh SY, Liu C, Liu J, Clark AG, Messer PW. PLoS Genet. 2017 Jul 20;13(7):e1006796. doi: 10.1371/journal.pgen.1006796. eCollection 2017 Jul. PubMed
Increased acetylation of microtubules rescues human tau-induced microtubule defects and neuromuscular junction abnormalities in Drosophila. Mao CX, Wen X, Jin S, Zhang YQ. Dis Model Mech. 2017 Oct 1;10(10):1245-1252. doi: 10.1242/dmm.028316. Epub 2017 Aug 17. PubMed
CRISPR/Cas9 and active genetics-based trans-species replacement of the endogenous Drosophila kni-L2 CRM reveals unexpected complexity. Xu XS, Gantz VM, Siomava N, Bier E. Elife. 2017 Dec 23;6. pii: 30281. doi: 10.7554/eLife.30281. PubMed
A postsynaptic PI3K-cII dependent signaling controller for presynaptic homeostatic plasticity. Hauswirth AG, Ford KJ, Wang T, Fetter RD, Tong A, Davis GW. Elife. 2018 Jan 5;7. pii: 31535. doi: 10.7554/eLife.31535. PubMed
The bHLH Protein Nulp1 is Essential for Femur Development Via Acting as a Cofactor in Wnt Signaling in Drosophila. Zeng Q, Wan Y, Zhu P, Zhao M, Jiang F, Chen J, Tang M, Zhu X, Li Y, Zha H, Wang Y, Hu M, Mo X, Zhang Y, Chen Y, Chen Y, Ye X, Bodmer R, Ocorr K, Jiang Z, Zhuang J, Yuan W, Wu X. Curr Mol Med. 2018 Mar 9;17(7):509-517. doi: 10.2174/1566524018666180212145714. PubMed
Mechanical strain regulates the Hippo pathway in Drosophila. Fletcher GC, Diaz-de-la-Loza MD, Borreguero-Munoz N, Holder M, Aguilar-Aragon M, Thompson BJ. Development. 2018 Mar 8;145(5). pii: dev.159467. doi: 10.1242/dev.159467. PubMed
Visualization of Transvection in Living Drosophila Embryos. Lim B, Heist T, Levine M, Fukaya T. Mol Cell. 2018 Apr 19;70(2):287-296.e6. doi: 10.1016/j.molcel.2018.02.029. Epub 2018 Mar 29. PubMed
Ari-1 Regulates Myonuclear Organization Together with Parkin and Is Associated with Aortic Aneurysms. Tan KL, Haelterman NA, Kwartler CS, Regalado ES, Lee PT, Nagarkar-Jaiswal S, Guo DC, Duraine L, Wangler MF, Bamshad MJ, Nickerson DA, Lin G, Milewicz DM, Bellen HJ. Dev Cell. 2018 Apr 23;45(2):226-244.e8. doi: 10.1016/j.devcel.2018.03.020. PubMed
Reducing resistance allele formation in CRISPR gene drive. Champer J, Liu J, Oh SY, Reeves R, Luthra A, Oakes N, Clark AG, Messer PW. Proc Natl Acad Sci U S A. 2018 May 22;115(21):5522-5527. doi: 10.1073/pnas.1720354115. Epub 2018 May 7. PubMed
The Drosophila homologue of MEGF8 is essential for early development. Lloyd DL, Toegel M, Fulga TA, Wilkie AOM. Sci Rep. 2018 Jun 8;8(1):8790. doi: 10.1038/s41598-018-27076-y. PubMed
Gorab is a Golgi protein required for structure and duplication of Drosophila centrioles. Kovacs L, Chao-Chu J, Schneider S, Gottardo M, Tzolovsky G, Dzhindzhev NS, Riparbelli MG, Callaini G, Glover DM. Nat Genet. 2018 Jul;50(7):1021-1031. doi: 10.1038/s41588-018-0149-1. Epub 2018 Jun 11. PubMed
gamma-TuRC Heterogeneity Revealed by Analysis of Mozart1. Tovey CA, Tubman CE, Hamrud E, Zhu Z, Dyas AE, Butterfield AN, Fyfe A, Johnson E, Conduit PT. Curr Biol. 2018 Jul 23;28(14):2314-2323.e6. doi: 10.1016/j.cub.2018.05.044. Epub 2018 Jul 5. PubMed
An expanded toolkit for gene tagging based on MiMIC and scarless CRISPR tagging in Drosophila. Li-Kroeger D, Kanca O, Lee PT, Cowan S, Lee MT, Jaiswal M, Salazar JL, He Y, Zuo Z, Bellen HJ. Elife. 2018 Aug 9;7. pii: 38709. doi: 10.7554/eLife.38709. PubMed
Behavior of homing endonuclease gene drives targeting genes required for viability or female fertility with multiplexed guide RNAs. Oberhofer G, Ivy T, Hay BA. Proc Natl Acad Sci U S A. 2018 Oct 2;115(40):E9343-E9352. doi: 10.1073/pnas.1805278115. Epub 2018 Sep 17. PubMed
Molecular Interface of Neuronal Innate Immunity, Synaptic Vesicle Stabilization, and Presynaptic Homeostatic Plasticity. Harris N, Fetter RD, Brasier DJ, Tong A, Davis GW. Neuron. 2018 Dec 5;100(5):1163-1179.e4. doi: 10.1016/j.neuron.2018.09.048. Epub 2018 Oct 18. PubMed
Investigation of the Developmental Requirements of Drosophila HP1 and Insulator Protein Partner, HIPP1. Glenn SE, Geyer PK. G3 (Bethesda). 2019 Feb 7;9(2):345-357. doi: 10.1534/g3.118.200705. PubMed
Dynamic multifactor hubs interact transiently with sites of active transcription in Drosophila embryos. Mir M, Stadler MR, Ortiz SA, Hannon CE, Harrison MM, Darzacq X, Eisen MB. Elife. 2018 Dec 27;7. pii: 40497. doi: 10.7554/eLife.40497. PubMed
Molecular safeguarding of CRISPR gene drive experiments. Champer J, Chung J, Lee YL, Liu C, Yang E, Wen Z, Clark AG, Messer PW. Elife. 2019 Jan 22;8. pii: 41439. doi: 10.7554/eLife.41439. PubMed
Cleave and Rescue, a novel selfish genetic element and general strategy for gene drive. Oberhofer G, Ivy T, Hay BA. Proc Natl Acad Sci U S A. 2019 Mar 26;116(13):6250-6259. doi: 10.1073/pnas.1816928116. Epub 2019 Feb 13. PubMed
Axin proteolysis by Iduna is required for the regulation of stem cell proliferation and intestinal homeostasis in Drosophila. Gultekin Y, Steller H. Development. 2019 Mar 15;146(6). pii: dev.169284. doi: 10.1242/dev.169284. PubMed
Tissue-specific (ts)CRISPR as an efficient strategy for in vivo screening in Drosophila. Meltzer H, Marom E, Alyagor I, Mayseless O, Berkun V, Segal-Gilboa N, Unger T, Luginbuhl D, Schuldiner O. Nat Commun. 2019 May 8;10(1):2113. doi: 10.1038/s41467-019-10140-0. PubMed
PLEKHA4/kramer Attenuates Dishevelled Ubiquitination to Modulate Wnt and Planar Cell Polarity Signaling. Shami Shah A, Batrouni AG, Kim D, Punyala A, Cao W, Han C, Goldberg ML, Smolka MB, Baskin JM. Cell Rep. 2019 May 14;27(7):2157-2170.e8. doi: 10.1016/j.celrep.2019.04.060. PubMed
Strip and Cka negatively regulate JNK signalling during Drosophila spermatogenesis. La Marca JE, Diepstraten ST, Hodge AL, Wang H, Hart AH, Richardson HE, Somers WG. Development. 2019 Jun 28;146(13). pii: dev.174292. doi: 10.1242/dev.174292. PubMed
Parental allele-specific protein expression in single cells In vivo. Lo CA, Chen BE. Dev Biol. 2019 Jun 11. pii: S0012-1606(19)30221-0. doi: 10.1016/j.ydbio.2019.06.004. PubMed
piRNA-guided co-transcriptional silencing coopts nuclear export factors. Fabry MH, Ciabrelli F, Munafo M, Eastwood EL, Kneuss E, Falciatori I, Falconio FA, Hannon GJ, Czech B. Elife. 2019 Jun 20;8. pii: 47999. doi: 10.7554/eLife.47999. PubMed
PI31 Is an Adaptor Protein for Proteasome Transport in Axons and Required for Synaptic Development. Liu K, Jones S, Minis A, Rodriguez J, Molina H, Steller H. Dev Cell. 2019 Aug 19;50(4):509-524.e10. doi: 10.1016/j.devcel.2019.06.009. Epub 2019 Jul 18. PubMed
Casein kinase 1 family proteins promote Slimb-dependent Expanded degradation. Fulford AD, Holder MV, Frith D, Snijders AP, Tapon N, Ribeiro PS. Elife. 2019 Sep 30;8. pii: 46592. doi: 10.7554/eLife.46592. PubMed
Genome editing retraces the evolution of toxin resistance in the monarch butterfly. Karageorgi M, Groen SC, Sumbul F, Pelaez JN, Verster KI, Aguilar JM, Hastings AP, Bernstein SL, Matsunaga T, Astourian M, Guerra G, Rico F, Dobler S, Agrawal AA, Whiteman NK. Nature. 2019 Oct;574(7778):409-412. doi: 10.1038/s41586-019-1610-8. Epub 2019 Oct 2. PubMed
F654A and K558Q Mutations in NMDA Receptor 1 Affect Ethanol-Induced Behaviors in Drosophila. Troutwine B, Park A, Velez-Hernandez ME, Lew L, Mihic SJ, Atkinson NS. Alcohol Clin Exp Res. 2019 Dec;43(12):2480-2493. doi: 10.1111/acer.14215. Epub 2019 Nov 1. PubMed
The Hippo pathway integrates PI3K-Akt signals with mechanical and polarity cues to control tissue growth. Borreguero-Munoz N, Fletcher GC, Aguilar-Aragon M, Elbediwy A, Vincent-Mistiaen ZI, Thompson BJ. PLoS Biol. 2019 Oct 15;17(10):e3000509. doi: 10.1371/journal.pbio.3000509. eCollection 2019 Oct. PubMed
Peptidoglycan-dependent NF-kappaB activation in a small subset of brain octopaminergic neurons controls female oviposition. Masuzzo A, Maniere G, Viallat-Lieutaud A, Avazeri E, Zugasti O, Grosjean Y, Kurz CL, Royet J. Elife. 2019 Oct 29;8. pii: 50559. doi: 10.7554/eLife.50559. PubMed
Glycogen branching enzyme controls cellular iron homeostasis via Iron Regulatory Protein 1 and mitoNEET. Huynh N, Ou Q, Cox P, Lill R, King-Jones K. Nat Commun. 2019 Nov 29;10(1):5463. doi: 10.1038/s41467-019-13237-8. PubMed
Dopamine modulates the optomotor response to unreliable visual stimuli in Drosophila melanogaster. Akiba M, Sugimoto K, Aoki R, Murakami R, Miyashita T, Hashimoto R, Hiranuma A, Yamauchi J, Ueno T, Morimoto T. Eur J Neurosci. 2020 Feb;51(3):822-839. doi: 10.1111/ejn.14648. Epub 2019 Dec 30. PubMed
Use of the CRISPR-Cas9 System in Drosophila Cultured Cells to Introduce Fluorescent Tags into Endogenous Genes. Bosch JA, Knight S, Kanca O, Zirin J, Yang-Zhou D, Hu Y, Rodiger J, Amador G, Bellen HJ, Perrimon N, Mohr SE. Curr Protoc Mol Biol. 2020 Mar;130(1):e112. doi: 10.1002/cpmb.112. PubMed
Rho-Kinase Planar Polarization at Tissue Boundaries Depends on Phospho-regulation of Membrane Residence Time. Sidor C, Stevens TJ, Jin L, Boulanger J, Roper K. Dev Cell. 2020 Feb 10;52(3):364-378.e7. doi: 10.1016/j.devcel.2019.12.003. Epub 2020 Jan 2. PubMed
Tissue-Scale Mechanical Coupling Reduces Morphogenetic Noise to Ensure Precision during Epithelial Folding. Eritano AS, Bromley CL, Bolea Albero A, Schutz L, Wen FL, Takeda M, Fukaya T, Sami MM, Shibata T, Lemke S, Wang YC. Dev Cell. 2020 Apr 20;53(2):212-228.e12. doi: 10.1016/j.devcel.2020.02.012. Epub 2020 Mar 12. PubMed
Nucleolar stress in Drosophila neuroblasts, a model for human ribosomopathies. Baral SS, Lieux ME, DiMario PJ. Biol Open. 2020 Apr 13;9(4). pii: bio.046565. doi: 10.1242/bio.046565. PubMed
Notch Mediates Inter-tissue Communication to Promote Tumorigenesis. Boukhatmi H, Martins T, Pillidge Z, Kamenova T, Bray S. Curr Biol. 2020 May 18;30(10):1809-1820.e4. doi: 10.1016/j.cub.2020.02.088. Epub 2020 Apr 9. PubMed
Intimate functional interactions between TGS1 and the Smn complex revealed by an analysis of the Drosophila eye development. Maccallini P, Bavasso F, Scatolini L, Bucciarelli E, Noviello G, Lisi V, Palumbo V, D'Angeli S, Cacchione S, Cenci G, Ciapponi L, Wakefield JG, Gatti M, Raffa GD. PLoS Genet. 2020 May 26;16(5):e1008815. doi: 10.1371/journal.pgen.1008815. eCollection 2020 May. PubMed
High-Efficiency CRISPR/Cas9 Mutagenesis of the white Gene in the Milkweed Bug Oncopeltus fasciatus. Reding K, Pick L. Genetics. 2020 Aug;215(4):1027-1037. doi: 10.1534/genetics.120.303269. Epub 2020 Jun 3. PubMed
Tissue specific requirement of Drosophila Rcd4 for centriole duplication and ciliogenesis. Panda P, Kovacs L, Dzhindzhev N, Fatalska A, Persico V, Geymonat M, Riparbelli MG, Callaini G, Glover DM. J Cell Biol. 2020 Sep 7;219(8). pii: 151861. doi: 10.1083/jcb.201912154. PubMed
Humanization of Drosophila Galphao to Model GNAO1 Paediatric Encephalopathies. Savitsky M, Solis GP, Kryuchkov M, Katanaev VL. Biomedicines. 2020 Oct 6;8(10). pii: biomedicines8100395. doi: 10.3390/biomedicines8100395. PubMed
Magnesium efflux from Drosophila Kenyon cells is critical for normal and diet-enhanced long-term memory. Wu Y, Funato Y, Meschi E, Jovanoski KD, Miki H, Waddell S. Elife. 2020 Nov 26;9. pii: 61339. doi: 10.7554/eLife.61339. PubMed
A putative stem-loop structure in Drosophila crumbs is required for mRNA localisation in epithelia and germline cells. Bhagavatula S, Knust E. J Cell Sci. 2021 Jan 25;134(2). pii: jcs.236497. doi: 10.1242/jcs.236497. PubMed
Precise genome engineering in Drosophila using prime editing. Bosch JA, Birchak G, Perrimon N. Proc Natl Acad Sci U S A. 2021 Jan 5;118(1). pii: 2021996118. doi: 10.1073/pnas.2021996118. PubMed Associated Plasmids pCFD3-NS pCFD5-NS pEntr_PE2 pNos-PE2-attB pUAS-PE2-attB More ...
DNA polymerase theta suppresses mitotic crossing over. Carvajal-Garcia J, Crown KN, Ramsden DA, Sekelsky J. PLoS Genet. 2021 Mar 22;17(3):e1009267. doi: 10.1371/journal.pgen.1009267. eCollection 2021 Mar. PubMed
Cis-regulatory chromatin loops arise before TADs and gene activation, and are independent of cell fate during early Drosophila development. Espinola SM, Gotz M, Bellec M, Messina O, Fiche JB, Houbron C, Dejean M, Reim I, Cardozo Gizzi AM, Lagha M, Nollmann M. Nat Genet. 2021 Apr;53(4):477-486. doi: 10.1038/s41588-021-00816-z. Epub 2021 Apr 1. PubMed
Robo recruitment of the Wave regulatory complex plays an essential and conserved role in midline repulsion. Chaudhari K, Gorla M, Chang C, Kania A, Bashaw GJ. Elife. 2021 Apr 12;10. pii: 64474. doi: 10.7554/eLife.64474. PubMed
Role of Hakai in m(6)A modification pathway in Drosophila. Wang Y, Zhang L, Ren H, Ma L, Guo J, Mao D, Lu Z, Lu L, Yan D. Nat Commun. 2021 Apr 12;12(1):2159. doi: 10.1038/s41467-021-22424-5. PubMed
Secreted C-type lectin regulation of neuromuscular junction synaptic vesicle dynamics modulates coordinated movement. Bhimreddy M, Rushton E, Kopke DL, Broadie K. J Cell Sci. 2021 May 1;134(9). pii: 261954. doi: 10.1242/jcs.257592. Epub 2021 May 11. PubMed
CopyCatchers are versatile active genetic elements that detect and quantify inter-homolog somatic gene conversion. Li Z, Marcel N, Devkota S, Auradkar A, Hedrick SM, Gantz VM, Bier E. Nat Commun. 2021 May 11;12(1):2625. doi: 10.1038/s41467-021-22927-1. PubMed Associated Plasmids pUC19-pleCopyCatcher pUC19-whiteCopyCatcher
Highly Efficient Temperature Inducible CRISPR-Cas9 Gene Targeting in Drosophila suzukii. Yan Y, Kobayashi Y, Huang C, Liu B, Qian W, Wan F, Schetelig MF. Int J Mol Sci. 2021 Jun 23;22(13). pii: ijms22136724. doi: 10.3390/ijms22136724. PubMed
Maternally inherited piRNAs direct transient heterochromatin formation at active transposons during early Drosophila embryogenesis. Fabry MH, Falconio FA, Joud F, Lythgoe EK, Czech B, Hannon GJ. Elife. 2021 Jul 8;10. pii: 68573. doi: 10.7554/eLife.68573. PubMed
Torsin and NEP1R1-CTDNEP1 phosphatase affect interphase nuclear pore complex insertion by lipid-dependent and lipid-independent mechanisms. Jacquemyn J, Foroozandeh J, Vints K, Swerts J, Verstreken P, Gounko NV, Gallego SF, Goodchild R. EMBO J. 2021 Sep 1;40(17):e106914. doi: 10.15252/embj.2020106914. Epub 2021 Jul 27. PubMed
Generation and characterization of fruitless P1 promoter mutant in Drosophila melanogaster. Neville MC, Eastwood A, Allen AM, de Haan A, Nojima T, Goodwin SF. J Neurogenet. 2021 Sep;35(3):285-294. doi: 10.1080/01677063.2021.1931179. Epub 2021 Aug 2. PubMed
A cis-regulatory element promoting increased transcription at low temperature in cultured ectothermic Drosophila cells. Bai Y, Caussinus E, Leo S, Bosshardt F, Myachina F, Rot G, Robinson MD, Lehner CF. BMC Genomics. 2021 Oct 28;22(1):771. doi: 10.1186/s12864-021-08057-4. PubMed
Dissecting the evolutionary role of the Hox gene proboscipedia in Drosophila mouthpart diversification by full locus replacement. Auradkar A, Bulger EA, Devkota S, McGinnis W, Bier E. Sci Adv. 2021 Nov 12;7(46):eabk1003. doi: 10.1126/sciadv.abk1003. Epub 2021 Nov 10. PubMed
TM2D genes regulate Notch signaling and neuronal function in Drosophila. Salazar JL, Yang SA, Lin YQ, Li-Kroeger D, Marcogliese PC, Deal SL, Neely GG, Yamamoto S. PLoS Genet. 2021 Dec 14;17(12):e1009962. doi: 10.1371/journal.pgen.1009962. eCollection 2021 Dec. PubMed
Protein visualization and manipulation in Drosophila through the use of epitope tags recognized by nanobodies. Xu J, Kim AR, Cheloha RW, Fischer FA, Li JSS, Feng Y, Stoneburner E, Binari R, Mohr SE, Zirin J, Ploegh HL, Perrimon N. Elife. 2022 Jan 25;11. pii: 74326. doi: 10.7554/eLife.74326. PubMed Associated Plasmids pMT-HGP-v3-Nb127D01-hIgG pMT-HGP-v3-NbVHH05-hIgG pET-26b-Nb127D01-HA-His pET-26b-NbVHH05-HA-His pET-26b-Nb127D01-ALFA-His More ...
The control of transcriptional memory by stable mitotic bookmarking. Bellec M, Dufourt J, Hunt G, Lenden-Hasse H, Trullo A, Zine El Aabidine A, Lamarque M, Gaskill MM, Faure-Gautron H, Mannervik M, Harrison MM, Andrau JC, Favard C, Radulescu O, Lagha M. Nat Commun. 2022 Mar 4;13(1):1176. doi: 10.1038/s41467-022-28855-y. PubMed
Double-tap gene drive uses iterative genome targeting to help overcome resistance alleles. Bishop AL, Lopez Del Amo V, Okamoto EM, Bodai Z, Komor AC, Gantz VM. Nat Commun. 2022 May 9;13(1):2595. doi: 10.1038/s41467-022-29868-3. PubMed
Essential role of Cp190 in physical and regulatory boundary formation. Kaushal A, Dorier J, Wang B, Mohana G, Taschner M, Cousin P, Waridel P, Iseli C, Semenova A, Restrepo S, Guex N, Aiden EL, Gambetta MC. Sci Adv. 2022 May 13;8(19):eabl8834. doi: 10.1126/sciadv.abl8834. Epub 2022 May 13. PubMed
Intronic gRNAs for the Construction of Minimal Gene Drive Systems. Nash A, Capriotti P, Hoermann A, Papathanos PA, Windbichler N. Front Bioeng Biotechnol. 2022 May 12;10:857460. doi: 10.3389/fbioe.2022.857460. eCollection 2022. PubMed
Functional Studies of Genetic Variants Associated with Human Diseases in Notch Signaling-Related Genes Using Drosophila. Yang SA, Salazar JL, Li-Kroeger D, Yamamoto S. Methods Mol Biol. 2022;2472:235-276. doi: 10.1007/978-1-0716-2201-8_19. PubMed
Intact Drosophila central nervous system cellular quantitation reveals sexual dimorphism. Jiao W, Spreemann G, Ruchti E, Banerjee S, Vernon S, Shi Y, Stowers RS, Hess K, McCabe BD. Elife. 2022 Jul 8;11. pii: 74968. doi: 10.7554/eLife.74968. PubMed
Selective endocytosis controls slit diaphragm maintenance and dynamics in Drosophila nephrocytes. Lang K, Milosavljevic J, Heinkele H, Chen M, Gerstner L, Spitz D, Kayser S, Helmstadter M, Walz G, Kottgen M, Spracklen A, Poulton J, Hermle T. Elife. 2022 Jul 25;11:e79037. doi: 10.7554/eLife.79037. PubMed
A genetic strategy to measure insulin signaling regulation and physiology in Drosophila. Tsao DD, Chang KR, Kockel L, Park S, Kim SK. PLoS Genet. 2023 Feb 2;19(2):e1010619. doi: 10.1371/journal.pgen.1010619. eCollection 2023 Feb. PubMed
Parkinsonism mutations in DNAJC6 cause lipid defects and neurodegeneration that are rescued by Synj1. Jacquemyn J, Kuenen S, Swerts J, Pavie B, Vijayan V, Kilic A, Chabot D, Wang YC, Schoovaerts N, Corthout N, Verstreken P. NPJ Parkinsons Dis. 2023 Feb 4;9(1):19. doi: 10.1038/s41531-023-00459-3. PubMed
Systematic analysis of RhoGEF/GAP localizations uncovers regulators of mechanosensing and junction formation during epithelial cell division. di Pietro F, Osswald M, De Las Heras JM, Cristo I, Lopez-Gay J, Wang Z, Pelletier S, Gaugue I, Leroy A, Martin C, Morais-de-Sa E, Bellaiche Y. Curr Biol. 2023 Mar 13;33(5):858-874.e7. doi: 10.1016/j.cub.2023.01.028. Epub 2023 Feb 13. PubMed
DMKPs provide a generalizable strategy for studying genes required for reproduction or viability in nontraditional model organisms. Laursen WJ, Busby R, Sarkissian T, Chang EC, Garrity PA. Genetics. 2023 May 26;224(2):iyad057. doi: 10.1093/genetics/iyad057. PubMed
Excess glutamate release triggers subunit-specific homeostatic receptor scaling. Han Y, Goel P, Chen J, Perry S, Tran N, Nishimura S, Sanjani M, Chien C, Dickman D. Cell Rep. 2023 Jul 25;42(7):112775. doi: 10.1016/j.celrep.2023.112775. Epub 2023 Jul 11. PubMed
Changes in searching behaviour of CSL transcription complexes in Notch active conditions. Baloul S, Roussos C, Gomez-Lamarca M, Muresan L, Bray S. Life Sci Alliance. 2023 Dec 14;7(3):e202302336. doi: 10.26508/lsa.202302336. Print 2024 Mar. PubMed
Opposing roles of Fos, Raw, and SARM1 in the regulation of axonal degeneration and synaptic structure. Waller TJ, Collins CA. Front Cell Neurosci. 2023 Nov 30;17:1283995. doi: 10.3389/fncel.2023.1283995. eCollection 2023. PubMed
Modular vector assembly enables rapid assessment of emerging CRISPR technologies. McGee AV, Liu YV, Griffith AL, Szegletes ZM, Wen B, Kraus C, Miller NW, Steger RJ, Escude Velasco B, Bosch JA, Zirin JD, Viswanatha R, Sontheimer EJ, Goodale A, Greene MA, Green TM, Doench JG. Cell Genom. 2024 Mar 13;4(3):100519. doi: 10.1016/j.xgen.2024.100519. PubMed Associated Plasmids AA016 AA017 AA021 AA022 AA023 More ...
Inter-organ steroid hormone signaling promotes myoblast fusion via direct transcriptional regulation of a single key effector gene. Ruan ZR, Yu Z, Xing C, Chen EH. Curr Biol. 2024 Apr 8;34(7):1438-1452.e6. doi: 10.1016/j.cub.2024.02.056. Epub 2024 Mar 20. PubMed
Timeless noncoding DNA contains cell-type preferential enhancers important for proper Drosophila circadian regulation. Ma D, Ojha P, Yu AD, Araujo MS, Luo W, Keefer E, Diaz MM, Wu M, Joiner WJ, Abruzzi KC, Rosbash M. Proc Natl Acad Sci U S A. 2024 Apr 9;121(15):e2321338121. doi: 10.1073/pnas.2321338121. Epub 2024 Apr 3. PubMed
Targeting mosquito X-chromosomes reveals complex transmission dynamics of sex ratio distorting gene drives. Haber DA, Arien Y, Lamdan LB, Alcalay Y, Zecharia C, Krsticevic F, Yonah ES, Avraham RD, Krzywinska E, Krzywinski J, Marois E, Windbichler N, Papathanos PA. Nat Commun. 2024 Jun 11;15(1):4983. doi: 10.1038/s41467-024-49387-7. PubMed

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